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egm-2 bulletkit medium  (Lonza)


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    Lonza egm-2 bulletkit medium
    Egm 2 Bulletkit Medium, supplied by Lonza, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/egm-2+bulletkit+medium/egm+2+medium/pm40668060-38-20-24
    Average 90 stars, based on 1 article reviews
    egm-2 bulletkit medium - by Bioz Stars, 2026-10
    90/100 stars

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    Related Articles

    Cell Culture:

    Article Title: Endomucin regulates the endothelial cytoskeleton independent of VEGF
    Article Snippet: Primary hRECs (P3) were purchased from Cell Systems (Troisdorf, Germany) and were cultured on glass cover slips that were coated with 0.2% gelatin-PBS for 30 min at 37°C. hRECs were cultured in EGM-2 BulletKit medium (Lonza, Basel, Switzerland, #CC-3162) supplemented with 2% fetal bovine serum, 2 mM L-glutamine (Lonza, #17-605E), and 100 U/mL penicillin–100 μg/mL streptomycin (Lonza, #17-602E) and were maintained in incubator at 37 °C with 5% CO 2 .

    Article Title: LncRNA H19/miR-107 regulates endothelial progenitor cell pyroptosis and promotes flow recovery of lower extremity ischemia through targeting FADD.
    Article Snippet: Plated on Fibronetin (BD Biocoat, 356008) coated six-well plates, the PBMCs were cultured in EGM-2 BulletKit (endothelial basal medium and SingleQuots Kit) medium (Lonza, CC-3156 & CC-4176) containing 20 % FBS and kept at 37 ◦C in a 5 % CO2 and 85 % humidified incubator.

    Article Title: Employment of diverse in vitro systems for analyzing multiple aspects of disease, hereditary hemorrhagic telangiectasia (HHT)
    Article Snippet: After incubation with beads, CD31 + cells were immediately isolated using the MidiMACS Separator and Starting Kit (LS) (Miltenyi Biotec) and cultured in EGM-2 Bulletkit Medium (Lonza).

    Article Title: Inhibition of Endothelial HIF-1α by IDF-11774 Attenuates Retinal Neovascularization and Vascular Leakage.
    Article Snippet: Primary human umbilical vein endothelial cells (HUVECs) were obtained from Zhong Qiao Xin Zhou Biotechnology (Shanghai, China) and maintained in EGM-2 BulletKit Medium (#CC-3162; Lonza, Basel, Switzerland).

    Article Title: New Anti‐Angiogenic Therapy for Glioblastoma With the Anti‐Depressant Sertraline
    Article Snippet: To differentiate into TDECs, 005 cells were cultured in the EGM‐2 BulletKit medium (Lonza, Basel, Switzerland) under hypoxic conditions (1% O 2 ).

    Article Title: Cold stress–induced ferroptosis in liver sinusoidal endothelial cells determines liver transplant injury and outcomes
    Article Snippet: Briefly, isolated LSECs were cultured in EGM-2 BulletKit medium (Lonza) supplemented with 20 ng/mL mouse VEGF (R&D Systems, Bio-Techne).

    Article Title: Polyethylene Glycol Loxenatide Accelerates Diabetic Wound Healing by Downregulating Systemic Inflammation and Improving Endothelial Progenitor Cell Functions.
    Article Snippet: EPCs were cultured in EGM-2 BulletKit medium (Lonza, Basel, Switzerland) supplemented with recombinant human rhEGF, rhFGF-B, rhVEGF, rhIGF-1, ascorbic acid, heparin, 5% fetal bovine serum, and 1% penicillin/streptomycin.

    Article Title: Endomucin regulates the endothelial cytoskeleton independent of VEGF
    Article Snippet: EGM-2 BulletKit medium (500 ul) (Lonza, #CC-3162) supplemented with 5% fetal bovine serum (FBS) (Atlanta Biologicals), 2 mM L-glutamine (Lonza, #17-605E), and 100 U/mL penicillin–100 μg/mL streptomycin (Lonza, #17-602E) was added to each well and incubated at 37°C with 5% CO2 for 48 hr.

    Recombinant:

    Article Title: Endomucin regulates the endothelial cytoskeleton independent of VEGF
    Article Snippet: Primary hRECs (P3) were purchased from Cell Systems (Troisdorf, Germany) and were cultured on glass cover slips that were coated with 0.2% gelatin-PBS for 30 min at 37°C. hRECs were cultured in EGM-2 BulletKit medium (Lonza, Basel, Switzerland, #CC-3162) supplemented with 2% fetal bovine serum, 2 mM L-glutamine (Lonza, #17-605E), and 100 U/mL penicillin–100 μg/mL streptomycin (Lonza, #17-602E) and were maintained in incubator at 37 °C with 5% CO 2 .

    Article Title: LncRNA H19/miR-107 regulates endothelial progenitor cell pyroptosis and promotes flow recovery of lower extremity ischemia through targeting FADD.
    Article Snippet: Plated on Fibronetin (BD Biocoat, 356008) coated six-well plates, the PBMCs were cultured in EGM-2 BulletKit (endothelial basal medium and SingleQuots Kit) medium (Lonza, CC-3156 & CC-4176) containing 20 % FBS and kept at 37 ◦C in a 5 % CO2 and 85 % humidified incubator.

    Article Title: Employment of diverse in vitro systems for analyzing multiple aspects of disease, hereditary hemorrhagic telangiectasia (HHT)
    Article Snippet: After incubation with beads, CD31 + cells were immediately isolated using the MidiMACS Separator and Starting Kit (LS) (Miltenyi Biotec) and cultured in EGM-2 Bulletkit Medium (Lonza).

    Article Title: Inhibition of Endothelial HIF-1α by IDF-11774 Attenuates Retinal Neovascularization and Vascular Leakage.
    Article Snippet: Primary human umbilical vein endothelial cells (HUVECs) were obtained from Zhong Qiao Xin Zhou Biotechnology (Shanghai, China) and maintained in EGM-2 BulletKit Medium (#CC-3162; Lonza, Basel, Switzerland).

    Article Title: New Anti‐Angiogenic Therapy for Glioblastoma With the Anti‐Depressant Sertraline
    Article Snippet: To differentiate into TDECs, 005 cells were cultured in the EGM‐2 BulletKit medium (Lonza, Basel, Switzerland) under hypoxic conditions (1% O 2 ).

    Article Title: Cold stress–induced ferroptosis in liver sinusoidal endothelial cells determines liver transplant injury and outcomes
    Article Snippet: Briefly, isolated LSECs were cultured in EGM-2 BulletKit medium (Lonza) supplemented with 20 ng/mL mouse VEGF (R&D Systems, Bio-Techne).

    Article Title: Polyethylene Glycol Loxenatide Accelerates Diabetic Wound Healing by Downregulating Systemic Inflammation and Improving Endothelial Progenitor Cell Functions.
    Article Snippet: EPCs were cultured in EGM-2 BulletKit medium (Lonza, Basel, Switzerland) supplemented with recombinant human rhEGF, rhFGF-B, rhVEGF, rhIGF-1, ascorbic acid, heparin, 5% fetal bovine serum, and 1% penicillin/streptomycin.

    Article Title: Endomucin regulates the endothelial cytoskeleton independent of VEGF
    Article Snippet: EGM-2 BulletKit medium (500 ul) (Lonza, #CC-3162) supplemented with 5% fetal bovine serum (FBS) (Atlanta Biologicals), 2 mM L-glutamine (Lonza, #17-605E), and 100 U/mL penicillin–100 μg/mL streptomycin (Lonza, #17-602E) was added to each well and incubated at 37°C with 5% CO2 for 48 hr.

    Incubation:

    Article Title: Endomucin regulates the endothelial cytoskeleton independent of VEGF
    Article Snippet: Primary hRECs (P3) were purchased from Cell Systems (Troisdorf, Germany) and were cultured on glass cover slips that were coated with 0.2% gelatin-PBS for 30 min at 37°C. hRECs were cultured in EGM-2 BulletKit medium (Lonza, Basel, Switzerland, #CC-3162) supplemented with 2% fetal bovine serum, 2 mM L-glutamine (Lonza, #17-605E), and 100 U/mL penicillin–100 μg/mL streptomycin (Lonza, #17-602E) and were maintained in incubator at 37 °C with 5% CO 2 .

    Article Title: LncRNA H19/miR-107 regulates endothelial progenitor cell pyroptosis and promotes flow recovery of lower extremity ischemia through targeting FADD.
    Article Snippet: Plated on Fibronetin (BD Biocoat, 356008) coated six-well plates, the PBMCs were cultured in EGM-2 BulletKit (endothelial basal medium and SingleQuots Kit) medium (Lonza, CC-3156 & CC-4176) containing 20 % FBS and kept at 37 ◦C in a 5 % CO2 and 85 % humidified incubator.

    Article Title: Employment of diverse in vitro systems for analyzing multiple aspects of disease, hereditary hemorrhagic telangiectasia (HHT)
    Article Snippet: After incubation with beads, CD31 + cells were immediately isolated using the MidiMACS Separator and Starting Kit (LS) (Miltenyi Biotec) and cultured in EGM-2 Bulletkit Medium (Lonza).

    Article Title: Inhibition of Endothelial HIF-1α by IDF-11774 Attenuates Retinal Neovascularization and Vascular Leakage.
    Article Snippet: Primary human umbilical vein endothelial cells (HUVECs) were obtained from Zhong Qiao Xin Zhou Biotechnology (Shanghai, China) and maintained in EGM-2 BulletKit Medium (#CC-3162; Lonza, Basel, Switzerland).

    Article Title: New Anti‐Angiogenic Therapy for Glioblastoma With the Anti‐Depressant Sertraline
    Article Snippet: To differentiate into TDECs, 005 cells were cultured in the EGM‐2 BulletKit medium (Lonza, Basel, Switzerland) under hypoxic conditions (1% O 2 ).

    Article Title: Cold stress–induced ferroptosis in liver sinusoidal endothelial cells determines liver transplant injury and outcomes
    Article Snippet: Briefly, isolated LSECs were cultured in EGM-2 BulletKit medium (Lonza) supplemented with 20 ng/mL mouse VEGF (R&D Systems, Bio-Techne).

    Article Title: Polyethylene Glycol Loxenatide Accelerates Diabetic Wound Healing by Downregulating Systemic Inflammation and Improving Endothelial Progenitor Cell Functions.
    Article Snippet: EPCs were cultured in EGM-2 BulletKit medium (Lonza, Basel, Switzerland) supplemented with recombinant human rhEGF, rhFGF-B, rhVEGF, rhIGF-1, ascorbic acid, heparin, 5% fetal bovine serum, and 1% penicillin/streptomycin.

    Article Title: Endomucin regulates the endothelial cytoskeleton independent of VEGF
    Article Snippet: EGM-2 BulletKit medium (500 ul) (Lonza, #CC-3162) supplemented with 5% fetal bovine serum (FBS) (Atlanta Biologicals), 2 mM L-glutamine (Lonza, #17-605E), and 100 U/mL penicillin–100 μg/mL streptomycin (Lonza, #17-602E) was added to each well and incubated at 37°C with 5% CO2 for 48 hr.

    Isolation:

    Article Title: Endomucin regulates the endothelial cytoskeleton independent of VEGF
    Article Snippet: Primary hRECs (P3) were purchased from Cell Systems (Troisdorf, Germany) and were cultured on glass cover slips that were coated with 0.2% gelatin-PBS for 30 min at 37°C. hRECs were cultured in EGM-2 BulletKit medium (Lonza, Basel, Switzerland, #CC-3162) supplemented with 2% fetal bovine serum, 2 mM L-glutamine (Lonza, #17-605E), and 100 U/mL penicillin–100 μg/mL streptomycin (Lonza, #17-602E) and were maintained in incubator at 37 °C with 5% CO 2 .

    Article Title: LncRNA H19/miR-107 regulates endothelial progenitor cell pyroptosis and promotes flow recovery of lower extremity ischemia through targeting FADD.
    Article Snippet: Plated on Fibronetin (BD Biocoat, 356008) coated six-well plates, the PBMCs were cultured in EGM-2 BulletKit (endothelial basal medium and SingleQuots Kit) medium (Lonza, CC-3156 & CC-4176) containing 20 % FBS and kept at 37 ◦C in a 5 % CO2 and 85 % humidified incubator.

    Article Title: Employment of diverse in vitro systems for analyzing multiple aspects of disease, hereditary hemorrhagic telangiectasia (HHT)
    Article Snippet: After incubation with beads, CD31 + cells were immediately isolated using the MidiMACS Separator and Starting Kit (LS) (Miltenyi Biotec) and cultured in EGM-2 Bulletkit Medium (Lonza).

    Article Title: Inhibition of Endothelial HIF-1α by IDF-11774 Attenuates Retinal Neovascularization and Vascular Leakage.
    Article Snippet: Primary human umbilical vein endothelial cells (HUVECs) were obtained from Zhong Qiao Xin Zhou Biotechnology (Shanghai, China) and maintained in EGM-2 BulletKit Medium (#CC-3162; Lonza, Basel, Switzerland).

    Article Title: New Anti‐Angiogenic Therapy for Glioblastoma With the Anti‐Depressant Sertraline
    Article Snippet: To differentiate into TDECs, 005 cells were cultured in the EGM‐2 BulletKit medium (Lonza, Basel, Switzerland) under hypoxic conditions (1% O 2 ).

    Article Title: Cold stress–induced ferroptosis in liver sinusoidal endothelial cells determines liver transplant injury and outcomes
    Article Snippet: Briefly, isolated LSECs were cultured in EGM-2 BulletKit medium (Lonza) supplemented with 20 ng/mL mouse VEGF (R&D Systems, Bio-Techne).

    Article Title: Polyethylene Glycol Loxenatide Accelerates Diabetic Wound Healing by Downregulating Systemic Inflammation and Improving Endothelial Progenitor Cell Functions.
    Article Snippet: EPCs were cultured in EGM-2 BulletKit medium (Lonza, Basel, Switzerland) supplemented with recombinant human rhEGF, rhFGF-B, rhVEGF, rhIGF-1, ascorbic acid, heparin, 5% fetal bovine serum, and 1% penicillin/streptomycin.

    Article Title: Endomucin regulates the endothelial cytoskeleton independent of VEGF
    Article Snippet: EGM-2 BulletKit medium (500 ul) (Lonza, #CC-3162) supplemented with 5% fetal bovine serum (FBS) (Atlanta Biologicals), 2 mM L-glutamine (Lonza, #17-605E), and 100 U/mL penicillin–100 μg/mL streptomycin (Lonza, #17-602E) was added to each well and incubated at 37°C with 5% CO2 for 48 hr.



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    90
    Lonza endothelial cell growth medium-2 bulletkit (egm-2)
    Overview of the SVZ‐on‐a‐chip. A) The SVZ‐on‐a‐chip structure 24 h post‐seeding, showing distinct cellular compartments visualized via staining. The entire chip structure is shown (scale bar = 3000 µm) alongside a magnified maximum Z‐projection view (scale bar = 100 µm). Arrows indicate neural stem cells (NSCs), and triangles highlight human fetal astrocytes (HFAs) embedded in Matrigel, occupying the central channel. The right channel is lined with human brain <t>microvascular</t> endothelial cells (HBMECs), while the left channel contains human choroid plexus epithelial cells (HCPEpiC). B) Neural channel with HCPEpiC seeded along the central channel (scale bar = 200 µm). C) The central channel, highlighted by triangles, is shown in closer detail (scale bar = 200 µm). D) 3D view of the inner channel, showing cells stained with Nestin (gray). Astrocytes, marked by triangles, are stained red but lack Nestin expression (scale bar = 70 µm). E) Inner channel viewed from a different angle (scale bar = 70 µm). F) Vascular channel lined with HBMECs (scale bar = 70 µm). G) HBMECs fully covering the vascular channel (scale bar = 70 µm). H) A magnified maximum Z‐projection view of the SVZ‐on‐a‐chip 7 days after complete seeding, showing significant cellular growth (scale bar = 100 µm). Clusters of NSCs are observed invading both the neural and, predominantly, the vascular channel. I) Maximum Z‐projection view of Nestin staining in the middle channel (scale bar = 100 µm). J) Close‐up of DCX‐positive neural stem cells invading the vascular channel. DCX staining was used instead of Nestin because HBMECs also express Nestin (scale bar = 50 µm). SVZ: Subventricular Zone, NSCs: Neural Stem Cells, HFAs: Human Fetal Astrocytes, HBMECs: Human Brain Microvascular Endothelial Cells, HCPEpiC: Human Choroid Plexus Epithelial Cells, DCX: Doublecortin.
    Endothelial Cell Growth Medium 2 Bulletkit (Egm 2), supplied by Lonza, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/egm-2+bulletkit+medium/egm+2+medium/pmc12146038-39-0-7
    Average 90 stars, based on 1 article reviews
    endothelial cell growth medium-2 bulletkit (egm-2) - by Bioz Stars, 2026-10
    90/100 stars
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    egm-2 endothelial growth medium-2 bulletkit  (Lonza)
    90
    Lonza egm-2 endothelial growth medium-2 bulletkit
    Overview of the SVZ‐on‐a‐chip. A) The SVZ‐on‐a‐chip structure 24 h post‐seeding, showing distinct cellular compartments visualized via staining. The entire chip structure is shown (scale bar = 3000 µm) alongside a magnified maximum Z‐projection view (scale bar = 100 µm). Arrows indicate neural stem cells (NSCs), and triangles highlight human fetal astrocytes (HFAs) embedded in Matrigel, occupying the central channel. The right channel is lined with human brain <t>microvascular</t> endothelial cells (HBMECs), while the left channel contains human choroid plexus epithelial cells (HCPEpiC). B) Neural channel with HCPEpiC seeded along the central channel (scale bar = 200 µm). C) The central channel, highlighted by triangles, is shown in closer detail (scale bar = 200 µm). D) 3D view of the inner channel, showing cells stained with Nestin (gray). Astrocytes, marked by triangles, are stained red but lack Nestin expression (scale bar = 70 µm). E) Inner channel viewed from a different angle (scale bar = 70 µm). F) Vascular channel lined with HBMECs (scale bar = 70 µm). G) HBMECs fully covering the vascular channel (scale bar = 70 µm). H) A magnified maximum Z‐projection view of the SVZ‐on‐a‐chip 7 days after complete seeding, showing significant cellular growth (scale bar = 100 µm). Clusters of NSCs are observed invading both the neural and, predominantly, the vascular channel. I) Maximum Z‐projection view of Nestin staining in the middle channel (scale bar = 100 µm). J) Close‐up of DCX‐positive neural stem cells invading the vascular channel. DCX staining was used instead of Nestin because HBMECs also express Nestin (scale bar = 50 µm). SVZ: Subventricular Zone, NSCs: Neural Stem Cells, HFAs: Human Fetal Astrocytes, HBMECs: Human Brain Microvascular Endothelial Cells, HCPEpiC: Human Choroid Plexus Epithelial Cells, DCX: Doublecortin.
    Egm 2 Endothelial Growth Medium 2 Bulletkit, supplied by Lonza, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/egm-2+bulletkit+medium/egm+2+medium/pm40511641-44-15-20
    Average 90 stars, based on 1 article reviews
    egm-2 endothelial growth medium-2 bulletkit - by Bioz Stars, 2026-10
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    Overview of the SVZ‐on‐a‐chip. A) The SVZ‐on‐a‐chip structure 24 h post‐seeding, showing distinct cellular compartments visualized via staining. The entire chip structure is shown (scale bar = 3000 µm) alongside a magnified maximum Z‐projection view (scale bar = 100 µm). Arrows indicate neural stem cells (NSCs), and triangles highlight human fetal astrocytes (HFAs) embedded in Matrigel, occupying the central channel. The right channel is lined with human brain microvascular endothelial cells (HBMECs), while the left channel contains human choroid plexus epithelial cells (HCPEpiC). B) Neural channel with HCPEpiC seeded along the central channel (scale bar = 200 µm). C) The central channel, highlighted by triangles, is shown in closer detail (scale bar = 200 µm). D) 3D view of the inner channel, showing cells stained with Nestin (gray). Astrocytes, marked by triangles, are stained red but lack Nestin expression (scale bar = 70 µm). E) Inner channel viewed from a different angle (scale bar = 70 µm). F) Vascular channel lined with HBMECs (scale bar = 70 µm). G) HBMECs fully covering the vascular channel (scale bar = 70 µm). H) A magnified maximum Z‐projection view of the SVZ‐on‐a‐chip 7 days after complete seeding, showing significant cellular growth (scale bar = 100 µm). Clusters of NSCs are observed invading both the neural and, predominantly, the vascular channel. I) Maximum Z‐projection view of Nestin staining in the middle channel (scale bar = 100 µm). J) Close‐up of DCX‐positive neural stem cells invading the vascular channel. DCX staining was used instead of Nestin because HBMECs also express Nestin (scale bar = 50 µm). SVZ: Subventricular Zone, NSCs: Neural Stem Cells, HFAs: Human Fetal Astrocytes, HBMECs: Human Brain Microvascular Endothelial Cells, HCPEpiC: Human Choroid Plexus Epithelial Cells, DCX: Doublecortin.

    Journal: Advanced Science

    Article Title: Subventricular Zone‐on‐a‐Chip: A Model to Study Neurogenesis Disruption in Neonatal Intraventricular Hemorrhage

    doi: 10.1002/advs.202502145

    Figure Lengend Snippet: Overview of the SVZ‐on‐a‐chip. A) The SVZ‐on‐a‐chip structure 24 h post‐seeding, showing distinct cellular compartments visualized via staining. The entire chip structure is shown (scale bar = 3000 µm) alongside a magnified maximum Z‐projection view (scale bar = 100 µm). Arrows indicate neural stem cells (NSCs), and triangles highlight human fetal astrocytes (HFAs) embedded in Matrigel, occupying the central channel. The right channel is lined with human brain microvascular endothelial cells (HBMECs), while the left channel contains human choroid plexus epithelial cells (HCPEpiC). B) Neural channel with HCPEpiC seeded along the central channel (scale bar = 200 µm). C) The central channel, highlighted by triangles, is shown in closer detail (scale bar = 200 µm). D) 3D view of the inner channel, showing cells stained with Nestin (gray). Astrocytes, marked by triangles, are stained red but lack Nestin expression (scale bar = 70 µm). E) Inner channel viewed from a different angle (scale bar = 70 µm). F) Vascular channel lined with HBMECs (scale bar = 70 µm). G) HBMECs fully covering the vascular channel (scale bar = 70 µm). H) A magnified maximum Z‐projection view of the SVZ‐on‐a‐chip 7 days after complete seeding, showing significant cellular growth (scale bar = 100 µm). Clusters of NSCs are observed invading both the neural and, predominantly, the vascular channel. I) Maximum Z‐projection view of Nestin staining in the middle channel (scale bar = 100 µm). J) Close‐up of DCX‐positive neural stem cells invading the vascular channel. DCX staining was used instead of Nestin because HBMECs also express Nestin (scale bar = 50 µm). SVZ: Subventricular Zone, NSCs: Neural Stem Cells, HFAs: Human Fetal Astrocytes, HBMECs: Human Brain Microvascular Endothelial Cells, HCPEpiC: Human Choroid Plexus Epithelial Cells, DCX: Doublecortin.

    Article Snippet: DPBS minus Ca ++ and M g++ (14 190 144, Thermo Fischer, Waltham, Massachusetts, USA), DPBS with Ca ++ and Mg ++ (14 040 091, Thermo Fischer), Astrocyte Media (AM1801, ScienCell, Carlsbad, California, USA), TrypLE (12 604 013, Thermo Fischer), Attachment factor protein 1× (S006100, Thermo Fischer), EGM ‐2 MV Microvascular Endothelial Cell Growth Medium‐2 BulletKit (CC‐3202, Lonza, Basel, Switzerland), Endothelial Cell Growth Medium MV2 (C‐22022, PromoCell, Heidelberg, Germany), Epithelial Cell Medium (Innoprot, P60104 , Derio, Spain), poly‐L‐lysine (PLL) (Innoprot), DMEM: F12 Glutamax (31331‐028, Thermo Fischer), N2 supplement ( 17502‐048, Thermo Fischer), B27 serum free (11 530 536, Thermo Fischer), FGF (233‐FB, R&D Systems, MN, USA), EGF (E9644, Sigma Aldrich, Burlington, Massachusetts, USA), PLO (P3655, Sigma Aldrich), Laminin (L2020, Sigma Aldrich), Matrigel Growth Factor Reduced (GFR) Basement Membrane Matrix, LDEV‐free (354 230, Corning, Corning, New York, USA), Antibiotic‐Antimycotic (15 240 062, Themo Fischer), idenTx 3 Chip (Aim Biotech, Singapore), Transwells 0.4 μM microporous membrane (833 932 041, Sarstedt, Nümbrecht, Germany), High Pure RNA Isolation Kit (11 828 665 001, Roche, Basel, Switzerland), Clariom S Affymetrix Assay (902 927, Thermo Fischer), High‐capacity RNA‐to‐cDNA kit (4 387 406, Thermo Fischer), TaqMan probes (Applied Biosystems, California, USA), Fast Advanced Master Mix (4 444 557, Applied Biosystems), TRIzol (15 596 026, Thermo Fischer), Chloroform (194 002, MP Biomedicals, Santa Ana, California, USA), Cell recovery solution (354 253, Corning), Anti‐Adherence Rinsing Solution (0 7010, STEMCELL Technologies, Vancouver, Canada), AggreWell800 24‐well plates (34 811, STEMCELL Technologies), Goat serum (G9023, Sigma Aldrich), Trypan Blue (1 450 021, Bio‐Rad, Hercules, California, USA), LEGENDplex Human Inflammation Panel 1 (13‐plex) assay using a V‐bottom plate (BioLegend, San Diego, California, USA), CellROX Green reagent ( C10444 ,Invitrogen), JC‐1 dye (T3168, Invitrogen) IL1receptor antagonist (SRP3327, Sigma Aldrich).

    Techniques: Staining, Expressing